The performance characteristics achieved in the MAQC study were due to the presence of internal standards (ISs) within a Standardized Mixture of Internal Standards (SMIS[TM]) in every measurement.
Based on the MAQC study, the increased replicate variation at low expression levels was a consequence of natural phenomena, specifically platform-independent stochastic random sampling error (12).
In phase two, MAQC
researchers expect to spend two years determining whether various genomic "biomarkers" can reliably predict a disease's likely progression in a certain patient, and which drug should produce the safest and best treatment result.
For the MAQC
main study, seven microarray platforms were deployed at three independent test sites and five replicates were assayed at each site using commercially available standard reference total RNA samples.
National Center for Toxicological Research, FDA, is expected to present the results of the newly released MAQC
project during a keynote panel presentation on Monday, September 25 at 4:00 PM ET.
Stratagene's Universal Reference RNA was one of two high-quality reference standards selected as part of the MAQC
Participation in the MAQC
consortium demonstrates Asuragen's commitment to quality processes and results through its service offerings.
Reid stated, "The MAQC
project provides confidence in a variety of RNA expression technologies.
project successfully compared multiple whole-genome gene expression profiles across various commercial platforms at an unprecedented scale.
project represents a key step towards establishing the use of microarrays in the discovery, development and review of FDA-regulated products," said Robert Bondaryk, PhD, General Manager of Cogenics.
study has demonstrated that by practicing good scientific method in the laboratory, researchers can obtain accurate, reproducible data from microarrays," said Janet Warrington, Ph.
Leming Shi, computational chemist, National Center for Toxicological Research, FDA, and architect of the MAQC