MCF-7Breast Cancer Cell Line
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The MCF-7 human breast cancer cell line was obtained from the Cell Bank of Shanghai, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China).
MCF-7 cells were purchased from Shanghai Institute of Cell Biology.
On the basis of these observations, we reasoned that the MCF-7 human breast cancer cell proliferation (the E-Screen) assay (Soto et al.
A paclitaxel resistant cell line (MCF-7/PTX) was established from its isogenetic cell line MCF-7 by increasing the concentration paclitaxel in the culture medium in a stepwise manner over a period of 12 months, the condition of culture was same as MCF-7 cell line.
Human MCF-7 breast cancer cells and HepG2 liver cancer cells were purchased from the American Type Culture Collection (Manassas, VA).
Chemical structure of mollugin and characterization of MCF-7 and MCF-7/adr cells
We also examined the estrogenic and antiestrogenic activities using estrogen-responsive element (ERE)-luciferase reporter-transfected human breast cancer cell line MCF-7.
MCF-7 and MCF-7/ADR cells were grown in DMEM in a 5% C02 incubator at 37[degrees]C, supplemented with 10% fetal bovine serum and antibiotics (100[micro]g/ml penicillin and 100 jxg/ml streptomycin).
For this report, we examined the estrogenic activities of styrene oligomers in the presence or absence of a liver microsomal metabolic system using a yeast estrogen screening (YES) assay and an estrogen-responsive element (ERE)-luciferase reporter assay in the estrogen-responsive human breast cancer cell line MCF-7.
The MCF-7 mammary adenocarcinoma and CCD966SK fibroblast cell lines were obtained from the American Type Culture Collection and grown at 37 [degrees]C in RPMS-1640 and MEM media, respectively, supplemented with 10% fetal calf serum (FBS), and 100U/ml penicillin and 100 [mu]g/ml streptomycin (Invitrogen) in a humidified 5% [CO.
In the present study we have continued to examine the effects of triazine herbicides and several of their common metabolites on aromatase activity in several human cell lines--the H295R adrenocortical, JEG-3 placental, and MCF-7 breast cancer cell line.