On days 3 and 6 postinfection, we euthanized 6 mice, 3 ferrets, and 3 cats in each group for pathological analysis and virus titration in organs (by use of plaque assays in MDCK
After immunofluorescent analysis of stably-transfected MDCK
cells, we found that CPO co-localized with calnexin, an ER marker.
Drug-permeability and transporter assays in Caco-2 and MDCK
8) The concentration of GTP in MDCK
cells treated with ribavirin-MP was much lower than that with favipiravir-RMP.
Activity of Caspase-3 (an apoptotic marker) was absolutely interrelated with LPO whereas negatively interrelated with GSH in AFB1 treated MDCK
Cytotoxic effect of Q7G was tested on non-infected MDCK
Viability of MDCK
cells and HK-2 cells was determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich) assay after various treatments.
Comparative sensitivity of influenza A and RSV virus isolation in 3 different cell lines: MDCK
, HEp- 2 and MDCK
and HEp-2 were used in combination to determine the sensitivity for the propagation of both influenza and RSV and production of characteristics CPE.
and Vero cells for influenza virus vaccine production: a one-to-one comparison up to lab-scale bioreactor cultivation.
, MDBK, HeLa, and Vero are employed in the animal virology laboratories.
The most commonly used types of renal tubular cells for in vitro studies include the canine-derived MDCK
cells (a model of distal tubular cells), the porcine cell line LLC-PK1 (a model of proximal tubular cells), and the human HK-2 cell line.
Methods used include maintenance of a continuous cell culture of Marc145 swine cells and MDCK
cells for viral infections as well as [gamma]-irradiation of the viruses.