MECB (prepared in 1% CMC) was administered daily by oral gavage for 28 clays in single doses of 500 mg/kg (group I), 1000 mg/kg (group II) and 2000 mg/kg (group III) while the control rats (Group IV) received only the vehicle (1% CMC in distilled water).
923 min, respectively, while that of the MECB were 6.
In the acute 14-day toxicity study, the MECB, given at a close of 2000 mg/kg did not cause any visible signs of toxicity or mortality to the rats.
In sub-chronic 28-day toxicity study, no observable changes occurred in the general behaviours of the rats treated with different doses (500, 1000 and 2000 mg/kg) of MECB compared to the control group.
No lesions or pathological changes attributable to treatment with MECB were observed in the organs of either sex of the treated rats (data not shown), compared to their respective normal groups.
The toxicity of MECB in rats was evaluated by both acute 14-day and sub-chronic 28-day toxicological studies.
Therefore, the objectives of the chromatographic analyses were to determine the levels of coumarin and trans-cinnamaldehyde in the MECB before administration to the rats and to ensure the safe utilisation of Cinnamomum burmannii stem barks as spices and favouring agent.
Thus, this study indicated that the MECB produced little or no short-term effects on the treated rats.
In sub-chronic 28-day toxicity study, the rats were treated orally by gavage with the MECB at different doses (500, 1000 and 2000 mg/kg) daily for 28 days.
From the results obtained and analysed statistically, between the rats fed with MECB and the control group (Table 1) in either sex, no significant differences were observed in any of the selected parameters.
These data suggested that the MECB did not induce or cause any damage to the liver and kidneys of the rats and can be considered non-toxic to these animals.
The toxicology profile of MECB was further studied by histopathological analysis and microscopic examination on those isolated organs from both the treated and control rats.