mutation identified by mutational analysis of epidermal growth factor receptor signaling pathway genes in lung adenocarcinoma.
org/pdb); EGFR (PDB ID:), ART (PDB ID: 3E87), mTOR (PDP ID: 4JSP), MEK1
and MEK2 (PDB IDs: and respectively) and homology-modelled structure of human STAT3 created by us from murine crystal structure (PDB ID:) were set as the rigid receptor molecule.
5 [micro]M of the MEK1
[mitogen-activated protein kinase (MAPK)/ERK kinase 1] inhibitor U0126 (EMD Chemicals USA).
As the effects of Nc-5-s on LPS-induced cytokine secretion by THP-1 monocytes may in part be mediated through activation of the ERK1 /2 kinase, we investigated whether a known MEK1
/2 inhibitor, U0126, affected LPS-induced cytokine secretion by THP-1 cells in the same way as Nc-5-s.
NCCN recently received $4 million in the form of two research grants from GlaxoSmithKline to gain further insights into GSK2118436, a BRAF inhibitor, and GSK1120212, a MEK1
and MEK2 inhibitor, both promising therapies in the treatment of advanced melanoma.
6A), the same effect as the p38 MAPK inhibitor SB203580, the MEK1
/2 inhibitor PD098059 and the JNK inhibitor SP600125 (Fig.
Experiments with transfected cells that expressed constitutively activated MAPK kinase MEK1
and a specific inhibitor of p38 have shown that inactivation of ERKs and activation of p38 might be associated with the induction of apoptosis by arsenic trioxide (Iwama et al.
Arsenic trioxide (ATO) and MEK1
inhibition synergize to induce apoptosis in acute promyelocytic leukemia cells.
The protein expression levels of elements of different cascades related to cell cycle progression, like Akt/PKB, MEK1
/2, and ERK1 /2 were found to be changed as depicted in Fig.