The HPLC fingerprint of MEMAC fraction (stock solution 2.
For all experiments, the cells were suspended in fresh medium containing various concentrations of MEMAC or the equivalent volume of DMSO as a vehicle control.
5] per ml and incubated with various concentrations of MEMAC (0, 25, 50, 75, 100 [micro]g/ml) for indicated time point.
5]) were treated with or without varying concentrations of MEMAC for indicated time period.
5]cell/ml) were treated without or with MEMAC for 72h, and then harvested via centrifugation and incubated with an equal volume of 1% NBT dissolved in PBS, containing 200ng/ml of freshly diluted TPA (Sigma-Aldrich, St.
After treatment of HL60 cells with MEMAC for indicated time period, the cells were harvested and washed twice with ice-cold 1 x PBS, cell pellets were resuspended in extraction lysis buffer (50 mM HEPES, pH 7.
5] cells), and were treated with various doses of MEMAC for 24 h.
The HPLC fingerprint chromatogram was established for the quality control of MEMAC (Fig.
HL60 cells were treated with various concentrations of MEMAC for the indicated time points, cell viability was determined by the trypan blue dye exclusion test.
Abdel Moneim Mohamed al-Janahi, director at MEMAC
, said that the workshop was convened in accordance with decision of the 15th Ministerial Council of the Regional Organisation for the Protection of Marine Environment (ROPME), and it is an opportunity for participants to exchange information with international experts.
The law currently has to punish the violating ship as per the country it dumped the oil in, which in this case is most likely Iran," MEMAC director-general Captain Abdulmunem Janahi told the GDN.
However, the proposal needs approval from MEMAC member countries, which are all six GCC states, Iran and Iraq.