For all experiments, the cells were suspended in fresh medium containing various concentrations of MEMAC or the equivalent volume of DMSO as a vehicle control.
5] per ml and incubated with various concentrations of MEMAC (0, 25, 50, 75, 100 [micro]g/ml) for indicated time point.
5]) were treated with or without varying concentrations of MEMAC for indicated time period.
5]cell/ml) were treated without or with MEMAC for 72h, and then harvested via centrifugation and incubated with an equal volume of 1% NBT dissolved in PBS, containing 200ng/ml of freshly diluted TPA (Sigma-Aldrich, St.
After treatment of HL60 cells with MEMAC for indicated time period, the cells were harvested and washed twice with ice-cold 1 x PBS, cell pellets were resuspended in extraction lysis buffer (50 mM HEPES, pH 7.
5] cells), and were treated with various doses of MEMAC for 24 h.
The HPLC fingerprint chromatogram was established for the quality control of MEMAC (Fig.
HL60 cells were treated with various concentrations of MEMAC for the indicated time points, cell viability was determined by the trypan blue dye exclusion test.
MEMAC induces monocytic differentiation in HL60 cells
To investigate whether MEMAC was able to modulate the morphology of AML cells, cytospin preparations of treated cells were stained by Wright-Giemsa solution.
Additionally, treatment of HL60 cells with 75 [micro]g/m1 MEMAC for 72 h increased NSE activity compared with DMSO-treated controls from 8.
Incubation of HL60 cells with MEMAC showed a dose-dependent increase in expression of CD14 by flow cytometric analysis, whereas only a slight increase in CD11 b was observed (Fig.