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MESF |
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This paper describes the procedures used for assigning values of MESF (molecules of equivalent soluble fluorophore) to the microspheres with immobilized FITC. The present work uses fluorescein as the model fluorophore and points Out critical steps in the use of MESF (Molecules of Equivalent Soluble Fluorophores) values for quantitative flow cytometric measurements. The equality of fluorescence yields from two solutions leads to the notion of equivalent number of fluorophores in the two solutions that is the basis for assignment of MESF (Molecules of Equivalent Soluble Fluorophore) values. |
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