8) Comparable frequencies of E-cadherin expression in BSS (86%, n = 15 (21); 100%, n = 6 (8); 100%, n = 9 (7); and 100%, n = 9 (25)), and slightly higher frequency in MFSS (12%, n = 57 (21); 0 of 6 all weak, 1+ positive (25); 35%, n = 307; and 14%, n = 16 (8)) has been reported previously.
However, positive expression of GSK3 and pGSK3 was significantly correlated with down-regulated E-cadherin expression in MFSS (P < .
c) In MFSS, expression of dysadherin, Snail, Slug, Akt-p-Akt-PI3K and GSK3-p-GSK3 were significantly associated with the loss of E-cadherin expression.
21,22) Inactivation of E-cadherin, by gene mutations and/or promoter hypermethylation and Snail overexpression in the spindle tumor cells of MFSSs, suggest its development through a possible phenomenon of neoplastic EMT.
The SYT-SSX fusion gene status was also available in 6 BSSs (5 SYT-SSX1 and 1 SYT-SSX2 type) and 5 MFSSs (3 SYT-SSX2 and 2 SYT-SSX1).
In contrast to BSSs, significantly down-regulated expression of E-cadherin (1 of 27; 4%), and the catenins ([alpha], 1 of 27 [4%]; [beta], 3 of 27 [11%]; and [gamma], 1 of 27 [4%]) was observed in the MFSSs.
29) In MFSSs, more frequent expression of Snail (63%; n = 27) and Slug (67%; n = 27) was detected and was associated with significant down-regulation of E-cadherin (P < .
In the current study, dysadherin-positive immunostaining in MFSSs (52%; n = 27) was associated with significantly reduced expression of E-cadherin (P < .
43) In our study, although positive expression of Akt, pAkt, and PI3K was noted in both of the SS subtypes, the expression of Akt, pAkt, and PI3K was significantly associated with down-regulated E-cadherin expression in MFSSs (all P < .