These effects were significantly inhibited by treating cells with MGDG.
Effects of MGDG and a FASN inhibitor on the viability of SKBR-3 cells
CL-MGDG decreased cell viability more than commercial MGDG after 48 h.
MGDGs are present in a wide range of plants, and plant galactosyl lipids constitute approximately 80% of the membrane lipids, of which MGDG is the most abundant one and accounts for approximately 50% of the thylakoid lipids.
Here, we show that culturing SKBR-3 cells in a medium containing MGDG significantly decreased the levels of FASN, SCD, and SREBF1 mRNA (Fig.
Therefore, these results suggest that MGDG suppresses the expression of FASN through the similar mechanisms to ALA.
6, decrease in SKBR-3 cell viability was greater in treatment with MGDG than that with FASN inhibitor C75.
MGDG from spinach inhibits mammalian replicative DNA polymerase activity (Kuriyama et al.
MGDG was rapidly decomposed into FAs and its corresponding monogalactosylglycerols basically by lipase hydrolysis when exposed to pancreatic juice, suggesting that MGDG does not enter into the circulation as its intact form (Sugawara and Miyazawa 2000).
Further, it is suggested that MGDG significantly decreases the cell viability by inhibiting the expression of FASN as well as DNA polymerase activity of SKBR-3 cells, explaining the large decrease in cell viability in the presence of MGDG compared with that of C75 and that in cell line expressing FASN at high levels (Supplementary Fig.
fatty acid synthase; LXR, liver X receptor; LXRE, liver X receptor response element; MGDG, monogalactosyldiacylglycerol; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium; RXR, retinoid X receptor; SCD, stearoyl-CoA desaturase; SP1, stimulatory protein 1 site; SRE, sterol regulatory element; SREBF, sterol regulatory element binding transcription factor; SREBP, non-canonical sterol regulatory element binding protein recognition site.
4] cells per well), cells were treated with CL extracts, MGDGs, a-linolenic acid (ALA) and 5 [micro]M of the LXR agonist (GW3965) or 1 [micro]M of the RXR agonist (LG100268) for 24 h, washed with PBS, and cDNA was synthesized using a Gene Expression Cell-to-CT kit (Life Technologies, CA, USA).