The sensitivity and specificity and positive and negative predictive values (PPV and NPV) were calculated for the Xpert MTB/RIF assay and smear microscopy with a 95% CI using MGIT
culture as the gold standard.
Lowenstein-Jensen media or MGIT
DST results were available for 91.
Use of BACTEC MGIT
960 for recovery of mycobacteria from clinical specimens: multicenter study.
ABBREVIATIONS: AFB = acid-fast bacilli; CDC = Centers for Disease Control and Prevention; CSF = cerebrospinal fluid; DFA = direct fluorescent antibody; DNA = deoxyribonucleic acid; ELISA = enzyme-linked immunosorbent assay; FITC = fluorescein isothiocyanate; GIS = Geographical Information System; H1N1 = influenza A virus; LRN = Laboratory Response Network; MDS = Molecular Detection Services; MGIT
= mycobacteria growth indicator tube; MTB = Mycobacterium tuberculosis; PCR = polymerase chain reaction; PFGE = pulsed field gel electrophoresis; RNA = ribonucleic acid; STEC = shiga-toxin producing Escherichia coli; TB = tuberculosis; WHO = World Health Organization; WNV = West Nile virus
We undertook this study to compare MGIT
960 PZA susceptibility test with pyrazinamidase activity assays in 136 clinical isolates of M.
The WHO endorsed liquid culture systems as the standard of care for TB diagnosis and patient management following FIND demonstration studies on MGIT
culture and DST in eight countries.
5 000-10 000 for positive smear * Allows species identification and concomitant drug sensitivity testing (DST) Media * Solid media requires 6-8 weeks for confirmed diagnosis and further 4-6 weeks for DST * Liquid culture is faster with results of diagnosis and DST as soon as 7 days Growth detection methods * Radioactivity--BACTEC 460-TB[R] * Fluorescence--BACTEC MGIT
960[R] * Phage-based tests--FASTPlaque TB-RIF(tm) * Inverted microscopy--MODS Fig.
Accelerated detection and identification of mycobacteria with MGIT
960 and Cobas Amplicor systems.
The primary efficacy endpoint was the proportion of study subjects who achieved SCC by two months using the MGIT
The presence of the MDR TB strain was confirmed by the phenotypic antibiogram in a BBL MGIT
mycobacteria growth indicator tube (Becton Dickinson, Franklin Lakes, NJ, USA) after the isolate had been cultured.
Growth and Detection: BD BACTEC MGIT
Tubes tube 7ml
Industry heavyweights like Cisco, Ooredoo, Avaya and Microsoft in addition to GBM, Omantel, MGIT
and Oman Fibre Optics have supported COMEX 2015 as key sponsors.