KKXX-like motif, KALY, a probable ER (endoplasmic reticulum) Membrane Retention Signal, was found in the C-terminus of Chicken MGST1 peptides.
The 12 bp deletion/ insertion polymorphism was not found in genomic DNA (data not shown), which indicated that the 12 nt deletion-insertion in chicken MGST1 mRNA was not due to the mutation of DNA and it must have resulted from other factors.
In further study, it would be interesting to determine if the 12 nt deletion/ insertion polymorphism in the 5' UTR of the MGST1 gene functions similarly.
Construction of phylogenetic tree of the orthologous peptides for the MGST1
A phylogenetic tree of the orthologous peptides for MGST1 was constructed based on their homology in amino acid sequence using two kinds of drosophila MGST1 as the outgroup (Figure 5).
Expression analysis of MGST1 mRNA in Dwarf chickens
The expression profiles of the chicken MGST1 genes were examined by real-time PCR.
2004) reported that the expression of human and murine MGST1 mRNA was also the highest in liver.
In summary, the current study carried out the molecular cloning of chicken MGST1, the identification of its splice variants and the expression analysis of MGST1 mRNA in Dwarf Brown chickens for the first time and provided a base for the study of its biological function.