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MI3 |
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CPA, GRAND VALLEY STATE UNIVERSITY, GRAND RAPIDS, MI3 The amplicons were cloned into pGEM-T (Promega), and sequencing was conducted with MI3 primers by Lark Technologies (Essex, U. All 340 isolates were typed by PCR fingerprinting by using the minisatellite-specific oligonucleotide MI3 as a single primer and RFLP analysis of the URA5 gene with the restriction enzymes Sau96I and HhaI in a double digest. |
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