DEX induced inhibition of glucose uptake and the expression of insulin signaling markers GLUT4 and PI3K were found to be restored by 3[beta]-taraxerol and MIEE, thus delineating its mechanism of action in the reversal of insulin resistance.
To gain additional insight into the mechanism by which DEX induces insulin resistance and the pathway undertaken by MIEE and 3[beta]-taraxerol to reverse the insulin resistance; a detailed protein profiling was performed for the major markers involved in insulin signaling which was compared with the classical insulin sensitive model (uninduced 3T3L1 adipocytes).
Effect of MIEE and 3[beta]-taraxerol on glucose uptake on
The effect of MIEE and 3[beta]-taraxerol on glucose uptake in insulin-sensitive (uninduced 3T3L1 adipocytes) (Sangeetha et al.
Likewise, MIEE and 3[beta]-taraxerol were assessed for their cytotoxic effect on the insulin-sensitive (uninduced) and insulin-resistant (DEX induced) model using MIT reagent as described by Gayathri et al.
MIEE, 3[beta]-taraxerol and rosiglitazone treated insulin-sensitive and insulin-resistant cell lysates were prepared (Sangeetha et al.
Comparative assessment of the glucose uptake potential of MIEE and 3f3-taraxerol on the insulin-sensitive and insulin-resistant model of 3T3L1 adipocytes
To elucidate the role of MIEE and 3[beta]-taraxerol on reversal of insulin resistance, a dose response analysis was performed in an insulin-resistant model.
Effect of MIEE and 3[beta]-taraxerol on cytotoxicity in insulin sensitive and insulin resistant 3T3L1 adipocytes
The cytotoxic effect of MIEE and 3[beta]-taraxerol on both the models of study was assessed using cytotoxicity assay.
3] W J Laycock C Eng, MIEE
, Member IEEE "Adaptive Reclosure of HV Overhead Lines" IEEE transactions 1996 Rolls-Royce Industrial Power Group, Reyrolle Protection, Hebburn Tyne and Wear, NE31 1TZ, United Kingdom.