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MLST |
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MLST was performed on 46 isolates as described by Maiden et al. Primers used for MLST correspond to the protocol as described previously (8), with the exception of the forward primer for tpi; we used the sequence tpif 5'-GCAT TAGCAGATTTAGGCGT-3'. MLST profiles could not be determined in these cases because of the relatively poor quality and yield of DNA from the fixed tissue. |
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