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9% sodium chloride and then minced and diluted to a 10% (by weight) solution with 20 mmol of ice-cold Tris-hydrochloric acid buffer (pH: 7. 35) was measured in left ventricular whole homogenates diluted 1:20 (weight per volume) in extraction buffer, consisting of 40 mmol 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). 000 grams of cyclohexane, 360 grams of styrene and 720 grains of butadiene, followed by an addition of 20 mmol of TMEDA (tetramethylethylene diamine) and 13 mmol of n-BuLi to initiate a polymerization at 50 [degrees] C. |
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