| Acronym | Definition |
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| MLS | Multiple Listing Service (real estate) | | MLS | Major League Soccer | | MLS | Mills | | MLS | Metropolitan Library System (Oklahoma City, Oklahoma) | | MLS | Multi-Level Security | | MLS | Microwave Limb Sounder | | MLS | Microwave Landing System | | MLS | Mass Layoff Statistics (US Department of Labor) | | MLS | Maximum Length Sequence | | MLS | Multilevel Secure | | MLS | Multi-Layer Steel | | MLS | Medical Laboratory Science | | MLS | Master of Library and Information Science (degree) | | MLS | Michigan Lutheran Seminary (Saginaw, MI) | | MLS | Millilitres | | MLS | Microphthalmia with Linear Skin Defects | | MLS | Medium Long Shot (cinematography) | | MLS | Multiple Levels of Security | | MLS | Multi-Layer Switching | | MLS | Mobile Location Service | | MLS | Machine Learning System | | MLS | Minimal Language Skills (clinical settings to describe clients who lack communication skills) | | MLS | Miles City, MT, USA - Miles City (airport code) | | MLS | Minimum Living Standard | | MLS | Master Line State (Cisco) | | MLS | Mont-La-Salle (Ain Seadé- Beirut- Lebanon) | | MLS | Massive Loser Squad (Australia) | | MLS | Microwave Leasing Services (tower services firm located in Ohio, Indiana, West Virginia, North Carolina) | | MLS | Manuel Le Saux (Italian DJ) | | MLS | Microwave Scan Beam Landing System (NASA; space shuttle) | | MLS | Multi-Library Search | | MLS | Manitoba Land Surveyor | | MLS | Missile Launching System | | MLS | Modular Load System | | MLS | Music Library System | | MLS | Municipal Liquor Store | | MLS | Manistique and Lake Superior Railroad | | MLS | Mudline Suspension (oil and gas industry equipment) | | MLS | Member and Leader Services (LDS Church) | | MLS | Matched Load Simulation | | MLS | Michigan Leukemia Society | | MLS | Marine Load System | | MLS | Mid-Line Shift | | MLS | Multi-Level Segment | | MLS | Multi-Line Scan | | MLS | Multifrequency Laser Sounding | | MLS | Modified Lloyd Search |
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