| Acronym | Definition |
|---|
| MLS | Multiple Listing Service (real estate) |
| MLS | Major League Soccer |
| MLS | Metropolitan Library System (Oklahoma City, Oklahoma) |
| MLS | Mills |
| MLS | Multi-Layer Steel |
| MLS | Mass Layoff Statistics (US Department of Labor) |
| MLS | Medical Laboratory Science |
| MLS | Microwave Landing System |
| MLS | Multi-Level Security |
| MLS | Maximum Length Sequence |
| MLS | Microwave Limb Sounder |
| MLS | Millilitres |
| MLS | Multilevel Secure |
| MLS | Master of Library and Information Science (degree) |
| MLS | Michigan Lutheran Seminary (Saginaw, MI) |
| MLS | Medium Long Shot (cinematography) |
| MLS | Multi-Layer Switching |
| MLS | Microphthalmia with Linear Skin Defects |
| MLS | Multiple Levels of Security |
| MLS | Machine Learning System |
| MLS | Minimal Language Skills (clinical settings to describe clients who lack communication skills) |
| MLS | Mobile Location Service |
| MLS | Manuel Le Saux (Italian DJ) |
| MLS | Miles City, MT, USA - Miles City (airport code) |
| MLS | Minimum Living Standard |
| MLS | Mudline Suspension (oil and gas industry equipment) |
| MLS | Master Line State (Cisco) |
| MLS | Mont-La-Salle (Ain Seadé- Beirut- Lebanon) |
| MLS | Massive Loser Squad (Australia) |
| MLS | Microwave Leasing Services (tower services firm located in Ohio, Indiana, West Virginia, North Carolina) |
| MLS | Microwave Scan Beam Landing System (NASA; space shuttle) |
| MLS | Manitoba Land Surveyor |
| MLS | Modular Load System |
| MLS | Music Library System |
| MLS | Multi-Library Search |
| MLS | Municipal Liquor Store |
| MLS | Manistique and Lake Superior Railroad |
| MLS | Missile Launching System |
| MLS | Member and Leader Services (LDS Church) |
| MLS | Matched Load Simulation |
| MLS | Michigan Leukemia Society |
| MLS | Marine Load System |
| MLS | Mid-Line Shift |
| MLS | Multi-Level Segment |
| MLS | Montana Loon Society (conservation group) |
| MLS | Multi-Line Scan |
| MLS | Multifrequency Laser Sounding |
| MLS | Modified Lloyd Search |
| MLS | Maximum Linux Security (computing) |
| MLS | My Life Sucks |
| MLS | Managed Laboratory Service (various locations) |
| MLS | Major League Sniping (gaming clan) |
| MLS | Medicare Levy Surcharge (Australia) |