| Acronym | Definition |
|---|
| MOST | Ministry of Science and Technology |
| MOST | Museum of Science and Technology |
| MOST | Management of Social Transformations Programme |
| MOST | Microvariability and Oscillations of Stars (Canadian built orbiting telescope) |
| MOST | Media Oriented System Transport |
| MOST | Metal Oxide Semiconductor Transistor |
| MOST | Maynard Operation Sequence Technique |
| MOST | Molonglo Observatory Synthesis Telescope |
| MOST | Museum of South Texas History (formerly Hidalgo County Historical Museum; Edinburg, Texas) |
| MOST | MTL Open System Technologies |
| MOST | Multidisciplinary Optical Switching Technology |
| MOST | Mobile Open Systems Technologies |
| MOST | Marine Observing Systems Team |
| MOST | Maximizing Out-of-School Time |
| MOST | Media Oriented System Transfer |
| MOST | Mission Oriented Simulator Training |
| MOST | Mission Operations Support Team |
| MOST | Motorcycle Operator's Skill Test |
| MOST | Mobile Sonar Technology |
| MOST | Maintenance Optimization Support and Training |
| MOST | Multimedia On-Line Service Technologies (Israel) |
| MOST | Mosaic Optical Sensor Technology |
| MOST | Multipulse Observation Sizing Technique |
| MOST | Mobile Optical Surveillance Tracker |
| MOST | Mission Oriented System Tape |
| MOST | Modeling and Observing for Substitute Teaching |
| MOST | Monitoring Observation Surveillance Targeting (NATO, field teams in Bosnia and Herzegovina) |
| MOST | Metro Omaha Swim Team (Nebraska) |