| Acronym | Definition |
|---|
| MFS | Major Facilitator Superfamily |
| MFS | Ministerium für Staatssicherheit (former East German Security Ministry) |
| MFS | Metropolitan Fiber Systems (WorldCom) |
| MFS | Memory File System (OpenBSD) |
| MFS | Massachusetts Financial Services Company |
| MFS | Maine Forest Service |
| MFS | Macintosh File System |
| MFS | Marfan Syndrome |
| MFS | Microsoft Flight Simulator |
| MFS | Married Filing Separately |
| MFS | Monadnock Family Services |
| MFS | Mediterranean Forecasting System |
| MFS | Message Format Service |
| MFS | Metropolitan Fire Service (AU) |
| MFS | Maximum Frame Size (RFID firmware command) |
| MFS | Memory Foam System (Lowa boot-shafts) |
| MFS | Magnetic Tape Field Search |
| MFS | Monetary and Financial Statistics |
| MFS | Managed Firewall Services (secure internal networks) |
| MFS | Metal-Ferroelectric-Semiconductor |
| MFS | Manned Flight Simulator (US Navy man-in-the-loop simulation facility) |
| MFS | Mobile File Sync (IBM) |
| MFS | Mountain Farming Systems |
| MFS | multifunction switch (US DoD) |
| MFS | Modified Food Starch |
| MFS | Minix from Scratch |
| MFS | Model-Based Frame Scheduling |
| MFS | Middle Finger Salute |
| MFS | Modular Fuel System (US Army) |
| MFS | Multi Fuel System |
| MFS | Material Flow System |
| MFS | Metropolitan Fiber System |
| MFS | Manager of Financial Services |
| MFS | Multi-Functional Structure |
| MFS | Molecular Fluorescence Spectroscopy |
| MFS | Maquis Forces Ship (Maquis Forces International; Star Trek) |
| MFS | Multiphase Flow Separators |
| MFS | Macomb Family Services |
| MFS | Multiple Funding Solutions, Inc. |
| MFS | Missile Firing Station |
| MFS | Mean Field Strength |
| MFS | Monthly Financial Submission |
| MFS | Microfilarias (dog heartworm) |
| MFS | Marathon Finishing Systems |
| MFS | Moonfire Spam (gaming, World of Warcraft) |
| MFS | Multi-Function Spoiler (aviation) |