v] = voltage-gated potassium channel, MBP = myelin basic protein, MHC II = major histocompatibility class II, MS = multiple sclerosis, MuLV
= murine leukemia virus, NADPH = nicotinamide adenosine dinucleotide phosphate, NO = nitric oxide, NOS = NO synthase, PNS = peripheral nervous system, T cells = T lymphocytes.
Reverse transcription was performed using 500 ng of total RNA in the presence of random examers and MuLv
reverse transcriptase (Applera Corporation, USA).
Total RNA was collected using TRIzol reagent (Invitrogen, Carlsbad, CA) and purified with RNeasy Mini Kit columns (Qiagen, Valencia, CA) according to the manufacturer's protocol, and transcribed with MuLV
(Moloney murine leukemia virus) reverse transcriptase and oligo-dT primers.
Then 3 [micro]g RNA was reversed transcribed into cDNA in a final volume of 20 [micro]l containing 100 pmol random hexamer, and 50 U of MuLV
reverse transcriptase (New England Biolab) according to the manufacturer's guideline.
Purified total RNA was reverse-transcribed with MuLV
reverse transcriptase and oligo-dT primers.
5 U of MuLV
reverse transcriptase, and 20 U of RNase Inhibitor (Perkin-Elmer Applied Biosystems).
Purified RNA was reverse transcribed with MuLV
(murine leukemia virus) reverse transcriptase and oligo-dT primers.
Nevertheless, investigators have never detected FeLV, ALV, BLV, or natural MuLV
infection in humans (Klein 2002).
5 [micro]M random hexamer primers (Applied Biosystems), 20 units RNase inhibitor (Applied Biosystems), and 1 [micro]L (50 U) MuLV
reverse transcriptase (Applied Biosystems).
Total RNA was reverse transcribed with MuLV
reverse transcriptase and oligo-dT primers, then subjected to real-time PCR analysis using SYBR green PCR master mix (Applied Biosystems, Cheshire, UK).
5 [micro]g) was reverse transcribed using MuLV
(murine leukemia virus) reverse transcriptase (50 U) in 1 x PCR buffer (50 mM KCl and 10 mM Tris-HCl, pH 8.