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MuLV |
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Total RNA was reverse transcribed with MuLV reverse transcriptase and oligo-dT primers, then subjected to real-time PCR analysis using SYBR green PCR master mix (Applied Biosystems, Cheshire, UK). 5 [micro]M random hexamer primers (Applied Biosystems), 20 units RNase inhibitor (Applied Biosystems), and 1 [micro]L (50 U) MuLV reverse transcriptase (Applied Biosystems). 5 [micro]g) was reverse transcribed using MuLV (murine leukemia virus) reverse transcriptase (50 U) in 1 x PCR buffer (50 mM KCl and 10 mM Tris-HCl, pH 8. |
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