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| Acronym | Definition |
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| MFO | multinational force and observers (US DoD) | | MFO | Mathematisches Forschungsinstitut Oberwolfach (German: Oberwolfach Mathematical Research Institute; est. 1944) | | MFO | Mixed Function Oxidase | | MFO | Multi-Family Office | | MFO | Marine Fuel Oil | | MFO | Medium Fuel Oil | | MFO | Make Friends Online (dating website) | | MFO | Microfinance Organization | | MFO | Mazhar Fuat Özkan (band) | | MFO | Melbourne Film Office (Australia) | | MFO | Mr. Fixit Online (gamer site) | | MFO | Must Finish On (project management) | | MFO | Multiple Facility Organization | | MFO | Maine Film Office | | MFO | Multinational Force & Observers Medal | | MFO | Market Facilitation Organization | | MFO | Michael Fassbender Online (fansite) | | MFO | Multicast Fan-Out | | MFO | Multi-Function Oxidase (immunology) | | MFO | Maintenance Free Operations | | MFO | Materiel Fielding Operations | | MFO | Mandatory Family Outing | | MFO | Multi-Frequency - Originating (telephony) | | MFO | Maintenance Free Operating Period | | MFO | Master Frequency Oscillator | | MFO | Medical Flight Officer | | MFO | Michigan Film Office (Lansing, MI) | | MFO | Matratzen Factory Outlet (German: Factory Matress Outlet) | | MFO | Municipal Finance Officer (various locations) |
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