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MHC |
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Liu and colleagues (27) also examined myosin heavy chain (MyHC) protein content of the spindle fibers. It has been hypothesized that thyroid hormone, which translocates to the nucleus of the cardiac cells, may alter the expression of certain cardiac proteins--including myosin heavy chain and sarcoplasmic reticulum adenosine triphosphatase in the cardiac myocytes--and lead to contractile dysfunction. Nomarsky optics and immunohistochemical techniques with antibodies to the [alpha]2(IV) chain, myosin heavy chain, and GFP are being used to analyze the phenotypic defects exhibited by homozygous animals with a CBS mutation. |
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