NCAM1Cell Adhesion Molecule, Neural, 1
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We used transcript concentrations of marker genes GATA1, RUNX1, SPI1, and NCAM1, commonly involved in leukemogenesis, to study the need for stabilization of BM samples in a clinical setting of disease monitoring.
Q-RT-PCR was performed in a 1-step method with 1 ng total RNA by use of the Quantitect[R] SYBR[R] Green reverse-transcription (RT)-PCR Kit and Quantitect Primer Assays (Qiagen) for the detection of GATA1, RUNX1, NCAM1, and SPI1 and for control gene 18S rRNA.
We observed lower mean (SD) gene expression at P2 vs R2 for all genes: GATA1 70 (12)%, RUNX1 50 (11)%, NCAM1 41 (8)%, and SPI1 51 (6)%.