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Human airway epithelial cell lines A549 and NCI-H292 were maintained in RPMI 1640 supplemented with 10% FBS, 2 mM L-glutamine, 1 mM NEAA, 10 U/mL streptomycin/penicillin, and 10 [micro]g/mL gentamicin. Furthermore, unless mentioned otherwise, DMEM amended with 1% NEAA, 2 mM glutamine, and 150 mg/L gentamicin was used for exposure experiments. |
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