NESTROFTNaked Eye Single Tube Red Cell Osmotic Fragility Test
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Blood samples (2 ml of EDTA blood from NESTROF test and 3 ml of non-ox lood for MSAFP) were collected from a subgroup of women with known last menstrual period (LMP) between 13-16 wk of gestation for estimation of maternal serum alpha-foetoprotein (MSAFP) and NESTROFT test (Naked Eye Single Tube Osmotic Fragility Test) along with routine haemogram.
Laboratory investigations NESTROFT followed by HB A2 estimation and MSAFP were carried out in 2548 cases.