m] melting temperature; FVL, factor 5 Leiden; PT, prothrombin; HFE, hemochromatosis gene; FRET, fluorescence resonance energy transfer; MGB, minor groove binding; NFQ
, nonfluorescent quencher; and 6-FAM, 6-carboxyfluorescein.
TagMan MGB probes are more stable and display increased mismatch discrimination, and their signal-to-noise ratio is improved by an NFQ
in place of the fluorescent quencher dye 6-carboxytetramethylrhodamine (TAMRA) (25).
The sequence of the reporter probe was 5'-(FAM)TCTGCGTAGGCAATCC(NFQ
)-3' (FAM, 6-carboxyfluorescein; NFQ
, nonfluorescent quencher; complementary to nt 17,745-17,760).
MOMENTUM will provide eligible jobseekers with Access to a range of quality education and training projects Links to identified job vacancies and employers Work placement and post programme support Relevant industry and NFQ
(National Framework of Qualifications) Certification.