3) -- HLTF Helicase like transcription factor LAMA1 Laminin subunit alpha 1 NEUROG1 NEUROG1 Neurogenin 1 NGFR NGFR
Nerve growth factor receptor RARB RARB Retinoic acid receptor beta RXRG RXRG Retinoid X receptor gamma RYR2 RYR2 Ryanodine receptor 2 SDC2 SDC2 Syndecan 2 SEPT9 SEPT9 Septin 9 SFRP2 SFRP2 Secreted frizzled-related SOCS3 SOCS3 Suppressor of cytokine signaling 3 SOX17 SOX17 SRY-box 17 THBD THBD Thrombomodulin Transmembrane protein with TMEFF2 TMEFF2 EGF-like and two follista- tin-like domains 2 UCHL1 UCHL1 Ubiquitin C-torminal hydro-lase L1 VIM VIM Vimentin Gene (a) Chromosomal (region) location (a) ADHFE1 8q12.
Assays for markers TMEFF2 and NGFR were run in both studies.
The best-performing marker panel was the combination of SEPT9 and NGFR, with a slight but significant bias-corrected AUC improvement of 2% over SEPT9 alone (P = 0.
NGFR was selected despite its low methylation rates in CRC tissue samples because it showed the highest complementarity to SEPT9 in the multivariate tissue analysis.
A qualitative analysis indicated the most sensitive marker to be SEPT9, which amplified 69% of CRC plasma samples, followed by TMEFF2 (65%) and NGFR (51%) (Table 3).
A quantitative analysis demonstrated SEPT9 to have significantly higher concentrations of methylated DNA in plasma samples from CRC patients than NGFR (P <0.
After thorough rinsing in phosphate-buffered saline, biotinylated rabbit anti-mouse immunoglobulin (1:400, Dakopatts, Glostrup, Denmark) for NGFR staining and goat anti-rabbit immunoglobulin (1:400, Dakopatts) for GLUT-1 staining were applied for 60 minutes at room temperature.
In the control colon specimens, we noted strong NGFR staining of nerve fibers and ganglion cells of the submucosal and myenteric plexuses and of nerve fibers within the circular and longitudinal muscle layers.
In healthy control bowel and in ganglionic HD (Figure 1), the large extrinsic nerves of the serosa stained both for GLUT-1 (ringlike pattern) and NGFR (both perineurium and nerve stained).
11] Immunohistochemical demonstration of NGFR staining of the perineurium of both hypertrophic and extrinsic fibers provides additional evidence.