Plasma samples from healthy individuals and CRF and NICTH patients were subsequently analyzed, and the results are listed in Table 2.
When plasma extracts of patients with NICTH were subjected to acid gel filtration, IGF-II IMR was distributed among the same major molecular size fractions as found for normal plasma.
IGF-II EXPRESSION IN NICTH TUMOR TISSUE From two NICTH patients included in this study, tumor tissues were available for detection of (pro)-IGF-II mRNA and protein by in situ hybridization and immunohistochemistry, respectively.
The present study demonstrates that tumors associated with NICTH may exhibit excessive concentrations of IGF-II mRNA, which is in accordance with previous reports (10, 29).
We found markedly increased concentrations of total E(68-88) IMR in the plasma of NICTH patients.
Confirmatory to semiquantitative data obtained by acid gel filtration, in CRF plasma the pro-IGF-II E(68-88)/total E(68-88) IMR ratio, derived directly from measurements by ELISA and RIA, respectively, was low compared with values found for either normal or NICTH plasma.
30) could not detect pro-IGF-II E(138-156) IMR in sera from various NICTH patients by Western immunoblotting with a antibody specific for E(138-156).
As indicated by the present results, the quantitative detection of pro-IGF-II by ELISA can be applied in the definitive diagnosis and follow-up of NICTH.