5 mM glucose, 19 mM NaHCO3
, 25 mM Hepes, 100 IU/ml penicillin, and 20% (v/v) FCS.
Amounts of pepsin, HCl, and NaHCO3
in the bags; each bag was labeled as shown in the column listing the contents.
All chick lens experiments (n=6) were performed in the MEM 199 containing 26 mM NaHCO3
35 g/L of NaHCO3
, 100 mg/L streptomycin, 100 U/mL penicillin G, 1 [micro]g/ mL amphotericin B) and clarified by centrifugation.
To imitate the body sweat a salted solution is being prepared from NaOH and NaHCO3
, diluted in water with an addition of CH3-COOH.
6), 30 mM 2-mercaptoethanol, 5 mM phosphoenolpyruvate, 50 mM NaHCO3
5 mol/L NaHCO3
were incubated in a ratio of 1:2:1 at 37 [degrees]C for 24 h.
5 ml/min) of the following composition (in mM): NaCl 137, NaHCO3
The culture medium was modified Dulbecco's Eagle's reinforced medium (Gibco, Karlsruhe, Germany), supplemented with 15% fetal calf serum (Flow Laboratories, Meckenheim, Germany), NaHCO3
Hidroksikobalamin verilmesi, NaHCO3
uygulamasindan sonra olgu kademeli olarak duzeldi.