| Acronym | Definition |
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| NLS | Native Language Support | | NLS | National Longitudinal Surveys | | NLS | National Library of Scotland | | NLS | National Language Support (extended characters) | | NLS | Nuclear Localization Signal (medicine; nuclear transport) | | NLS | National Library Service | | NLS | National Library Service for the Blind and Physically Handicapped | | NLS | National Literacy Strategy | | NLS | oNLine System | | NLS | Nonlinear Least Squares | | NLS | Nonlinear Schrödinger | | NLS | Noxious Liquid Substances | | NLS | National Leadership Seminar (Boy Scouts of America) | | NLS | National Lifeguard Service (Canada) | | NLS | Natural Language Support | | NLS | National Launch System | | NLS | Network License Server | | NLS | Neu-Laxova Syndrome (genetic disorder) | | NLS | Neu-Laxova Syndrome | | NLS | Nassau Library System | | NLS | Natural Language System | | NLS | New Launch System | | NLS | Normed Linear Spaces | | NLS | National Land Service | | NLS | Noise Line State (Cisco) | | NLS | Novell Licensing System | | NLS | New Life Singers (Marietta, Georgia) | | NLS | Noise Like Signals | | NLS | Native Lan Service | | NLS | New Level Safety (Dartford, UK) | | NLS | No Line of Sight | | NLS | Network Lifecycle Services (IDC) | | NLS | National Linen Service Corporation | | NLS | National Local Service | | NLS | Non-Linear Suppression (Nortel) | | NLS | Next Line and Set prediction | | NLS | Neville Lovett School (UK) | | NLS | None Left Standing (band) |
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