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OADC |
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Mycobacterial growth indicator tubes (Becton Dickinson, Sparks, MD) containing both 10% OADC (oleic acid, albumin, dextrose, and catalase) (Becton Dickinson, Sparks, MD), and 100 [micro]L of PANTA Antimicrobic Supplement (Polymyxin B, Amphotericin B, Nalidixic acid, Trimethoprim, and Azlocillin) (Becton Dickinson) were injected with 500 [micro]L of decontaminated sputum sample according to the manufacturer's specifications. ulcerans strain S-WT (from the Centers for Disease Control and Prevention culture collection; Atlanta) was recovered from a 1-ml lyophilisate in 10% glycerol by incubation in 5 ml Middlebrook 7H9 with OADC supplement (Remel, Lenexa, KS) at 32. 1 ml was plated onto Middlebrook and Cohn 7H10 agar with OADC enrichment (Difco Laboratories, Detroit, MI), and the remainder was used to inject 40 ml of Middlebrook 7H9 broth with ADC enrichment (Difco). |
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