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| Acronym | Definition |
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| OSL | Open Source Lab | | OSL | Optically Stimulated Luminescence | | OSL | Office of Student Life | | OSL | Oregon State Library | | OSL | Ontario Safety League (est. 1913; Canada) | | OSL | Oriented Strand Lumber | | OSL | Order of St Luke the Physician | | OSL | Optical Stimulated Luminescence | | OSL | Order of Saint Luke | | OSL | Oud Strijders Legioen (Dutch) | | OSL | Open-Short-Load (electronic calibration) | | OSL | Oregon Short Line Railroad | | OSL | Optimum Stimulation Level (marketing) | | OSL | Oslo, Norway - Gardermoen (Airport Code) | | OSL | OnGameNet Star League (Korean pro-gaming competition) | | OSL | Orbiting Solar Laboratory | | OSL | Observed Safe Level | | OSL | Office of Space Launch (National Reconnaissance Office) | | OSL | Off Scale Low (NASA) | | OSL | Open Space Laboratory (Tsukuba Research Center, Japan) | | OSL | Oceanographic Systems Laboratory | | OSL | On Scene Leader | | OSL | Operational Safety Limit | | OSL | Outside Length | | OSL | Overlay Service Layer | | OSL | Operations Service Leader | | OSL | Officer of the Order of St. Lazarus | | OSL | Orbiting Space Laboratory | | OSL | Operational Site Layout | | OSL | Over the Side Launch (tube launch torpedo) | | OSL | Outdoor Stream Laboratory (Minnesota) | | OSL | Object Source Lighting (painting) | | OSL | Outstanding Leg (structural engineering) | | OSL | One Sexy Lady | | OSL | Office of Service Learning (various locations) | | OSL | On-Site Laboratory | | OSL | Our Savior Lutheran (various locations) | | OSL | Open Source License |
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