| Acronym | Definition |
|---|
| OSF | Oregon Shakespeare Festival (Ashland, Oregon) |
| OSF | Open Software Foundation |
| OSF | Old Spaghetti Factory (US restaurant chain) |
| OSF | Orthoptera Species File |
| OSF | Order of St. Francis (Catholic group) |
| OSF | Office of Space Flight |
| OSF | Operational Support Facility |
| OSF | Open Systems Foundation |
| OSF | Oil Stabilization Fund |
| OSF | Objective Simulation Framework (US DoD) |
| OSF | Offset Field |
| OSF | Operations Support Flight |
| OSF | Operations System Function (ANSI) |
| OSF | Off Side Front (automotive suspension) |
| OSF | Oxidation-induced Stacking Fault |
| OSF | Office of Strategic Finance |
| OSF | Occupiable Square Feet |
| OSF | Other Structures and Facilities |
| OSF | Ordinis Sancti Francisci (of the order of St Francis) |
| OSF | Open Software Forum |
| OSF | Ordnance Storage Facility |
| OSF | Office Systems Family |
| OSF | Franciscan Brothers or Sisters (religious order) |
| OSF | Organizational Structure Facility |
| OSF | Obtain Service From |
| OSF | Congregation of Servants of Holy Infancy Jesus (religious order) |
| OSF | Omega Sector Fleetyards (Halo gaming) |
| OSF | Oversubscription Factor (Cisco) |
| OSF | Oyster Strike Force |
| OSF | Ordered Statistics Filter |
| OSF | On-Site Familiarization |
| OSF | Office of School Facilities (various locations) |
| OSF | Our Stolen Future (Theo Colborn book) |
| OSF | Office of Sustainable Fisheries (National Oceanic and Atmospheric Administration) |
| OSF | Open Source Food (est. 2008) |
| OSF | Ordinateurs Sans Frontieres (French: Computers Without Borders) |
| OSF | Optimistes Sans Frontieres (French: Optimists Without Borders; Belgium) |