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The cat was humanely killed, and its brain was sent to the Onderstepoort Veterinary Institute for rabies testing. Samples were analyzed at the Istituto Zooprofilattico Sperimentale delle Venezie (Italy), except for samples from Egypt that were analyzed at the US Naval Medical Research Unit-3 (Egypt), samples from Kenya and Malawi which were analyzed at the Agricultural Research Council Onderstepoort Veterinary Institute (RSA), and samples from Tunisia which were analyzed at the Southeast Poultry Research Laboratory (USA). Lyssavirus antigen was detected by the standard fluorescent antibody test (FAT) (17), with modifications, by using a polyclonal fluorescein isothiocyanate--conjugated immunoglobulin (Rabies Unit, Onderstepoort Veterinary Institute, Pretoria, South Africa) that could detect all lyssavirus genotypes. |
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