| Acronym | Definition |
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| OST | Office of Science and Technology | | OST | Out of School Time (after-school care programs) | | OST | Original Sound Track | | OST | Office of the Special Trustee for American Indians | | OST | Office of the Secretary of Transportation | | OST | Official Soundtrack | | OST | Observatoire des Sciences et Techniques (French: science and technology observatory) | | OST | Office of Secure Transportation (US Department of Energy) | | OST | On Second Thought | | OST | Old Spanish Trail | | OST | Outer Space Treaty | | OST | Offline Store | | OST | Oblate School of Theology | | OST | Open Storage (software) | | OST | On-Screen Takeoff (construction planning) | | OST | Optimal Solutions and Technologies (Washington, DC) | | OST | Online Service Provider | | OST | Operations Support Team | | OST | Operational Sea Training | | OST | Open Skies Treaty | | OST | Order Ship Time | | OST | Office of Security Transition | | OST | Originating Station Treatment | | OST | Orthogonal Space-Time (coding scheme) | | OST | Optical Standards Tester (Carcadiant Systems) | | OST | Open Source Teaching | | OST | Operational Suitability Test | | OST | Oral Specimen Test | | OST | Order & Shipping Time | | OST | Odonata Survey of Texas | | OST | Operational Requirements Document Support Team | | OST | Observational Sequence Table | | OST | Objective, Strategy, Tactics (business/marketing) | | OST | OPTEC System Team | | OST | Optical Switch Technology | | OST | Over-Stress Test | | OST | Ormat Solar Turbine | | OST | Oscillating Sand Abrasion Test |
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