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PCR-RFLP |
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was determined by PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) and small subunit (SSU) rRNA genes using methods based on those described by Spano et al. Repeatability Turnaround [duplicates Method [days (rank)] (rank)] SSCP of nontranscribed 1 (1) 5/5 (1) ITS-region of SSU rDNA SSCP of transcribed region 1 (1) 5/5 (l) of SSU rDNA Sequencing of GP60 DNA 2 (2) 4/5 (2) PCR-RFLP of SSU rDNA 4 (3) 5/5 (1) Multilocus genotyping at 2 (2) 2/5 (3) 3 microsatellites Sequencing of HSP70 4 (3) 5/5 (l) Discrimination Typeability [no. Genotyping with PCR-RFLP identified the following species: B. |
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