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PCR-SSCP

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AcronymDefinition
PCR-SSCPPolymerase Chain Reaction–Single Strand Conformation Polymorphism


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Pneumocystis jirovecii genotyping by PCR-SSCP of 4 genomic regions * SSCP pattern Patient Date ITS1 26S mt26 [beta]-tub Index case-patient 3/27/02 A A, B# A#, B, C A, B# 1 7/26/02 A A, B# A A 2 10/30/02 A A, B# A A 3 12/3/02 A A, B# A A 4 2/7/03 A, B A#, B#, C B, C# B, C# 5 2/15/03 A A, B# C A 6 2/18/03 A, B# A, B# A C Patient P.
In addition, the dihydropteroate synthase (DHPS) genotype was determined by using PCR-SSCP as described (29).
Some patients were co-infected with two different P carinii types, as shown by PCR-SSCP multilocus typing method (patients 4, 5, 8, 11, and 13) or DHPS genotyping (patients 3 and 4).
 
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