PCRRFLPPolymerase Chain Reaction Followed by Restriction Fragment Length Polymorphism Assay
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However the present result allows us to report that PCRRFLP of the mitochondrial cytochrome b gene is a suitable alternative that can be applied to the detection of pork in processed meat in commercialized products such as meatball and sausage.
In our investigation the intersection of costa, subcosta with bifurcation of R2+3 was not compatible with PCRRFLP result except the samples of Hamadan, Yazd and Chabahar areas (Table 2, Fig.
vivax field isolates employing simple PCR or PCRRFLP methods for three antigen genes revealed 2-5 alleles at Pvgam-1 (21-23), 11-23 at Pvcsp (16,24) and 36 at Pvmsp-1 (16) using substantial number of field isolates (>100).
After the second in vitro maturation, nucleus morphology of oocytes with the PCRRFLP genotype of HSP70 K1 region were showed the percentages reaching the M that the CC genotype was .
An identical observation was obtained in our PCRRFLP assay for detection of the CYP3A4*1B polymorphism [A(-290)G] (2).
In recent years, molecular techniques such as PCRRFLP of the 18S rRNA gene (6), kDNA-PCR (9), fluorescent quantitative PCR (6) and mini exon PCR assay (10) are used to identify Leishmania infections both in experimentally infected and field-captured phlebotomine sandflies, and could be a useful tool in epidemiological studies and strategic planning for the control of human leishmaniasis.
A SNP (C1006G) of H-FABP gene was found in bovine by PCRRFLP and the effect of this SNP on tenderness was significant (Li et al.
PCRRFLP analysis of the Cryptosporidium wall protein (COWP) gene discriminates between C.
The HH-LLA assay as described involves the same amplification times and steps as PCRRFLP analysis.
Six single nucleotide polymorphisms in the bovine HMGA1 gene were detected and the allele frequencies of these SNPs were investigated using the PCRRFLP method in nine cattle breeds including Limousin, Simmental, Brown Swiss, Hereford, Angus, Charolais, Hanwoo, Brahman and Red Chittagong cattle.
The standard PCRRFLP method applied to a large number of nail samples, however, yielded occasional questionable electropherograms.
We analyzed 256 specimens with this reverse ASO hybridization assay and by PCRRFLP.