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We designed highly purified salt-free OliGold primers (Eurogentec, Seraing, Belgium) for the internal control gene hypoxanthine phosphoribosyhransferase 1 (HPRT1) and for target genes estrogen receptor-[alpha] (ESR1), progesterone receptor (PGR), PDZ domain containing 1 (PDZK1), and erb-b2 erythroblastic leukemia viral oncogene homolog 2 (ERBB2) using Roche Lightcycler software (Roche Diagnostics Belgium, Vilvoorde, Belgium). Unexpected modes of PDZ domain scaffolding revealed by structure of nNOS-syntrophin complex. Throughout 2001, AxCell made continued progress in mapping PDZ, SH3, and SH2 domain families. |
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