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PFGE |
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PFGE patterns were classified as identical, similar (differed by 1-3 bands), or distinct (differed by >4 bands) (10). Both PFGE and flow cytometry require similar sample preparation times, but even when sample preparation time was factored in, increasing the flow cytometry time to 8 hours, it was still faster than the 24 hours required by PFGE. PFGE is a sensitive means of separating DNA and detecting patterns, or fingerprints that can be stored in a database and rapidly searched to distinguish between strains of disease-causing organisms, such as Escherichia coli O157:H7, Salmonella, Shigella, Listeria, and Campylobacter. |
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