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PFGEPulsed-Field Gel Electrophoresis
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Three isolates of PFGE type A6 (TSY216, TSY241, and TSY421) were obtained during June 3-July 5, 2010, from 3 unrelated residents of a village near the Thailand-Myanmar border.
The DSS-VDH also assisted with shoreline and sanitary surveys to compare potential sources identified in that manner with sources classified by ARA and PFGE.
If we want to use PFGE for source tracking in a large watershed like the Santa Ana River, a very extensive DNA fingerprint library is needed, because our study shows that even a minor change in the DNA fingerprint can significantly affect clonal groupings," Ibekwe explains.
The process can be completed within 8 h with a discriminatory power comparable to that of PFGE (17).
PFGE was run in a CHEF-DRIII apparatus (Bio-Rad, Hercules, CA, USA) at 6V/cm for 22 h.
Genotypic characterization of Enterobacter sakazakii isolates by PFGE, BOX-PCR, and sequencing of the fliC gene.
Conclusions: The use of PFGE on our state's most common isolates provides additional confirmation that despite being linked by time of onset and location of residence, the majority of the human salmonellosis cases in our region are still sporadic.
Their isolates were not available for PFGE testing.
Each organism has its own particular fingerprint in PFGE, which [Jett] has shown [to be] true in his flow cytometer as well, so the ability to analyze that fingerprint quickly and with accuracy would be invaluable.
0004 PFGE type, PT8, and pansusceptibility to antimicrobial agents.
All positive Salmonella isolates submitted to the LBDHHS Public Health Lab (PHL) are strain typed and then sent to CDPH Microbial Diseases Laboratory (MDL) for PFGE analysis.