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PSQ |
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For sequencing according to the pyrosequencing technology, biotinylated PCR amplicons were immobilized onto streptavidin-coated magnetic beads and denatured to produce single-stranded DNA by using a PSQ 96 Sample Prep Tool (Biotage AB, Uppsala, Sweden). Pyrosequencing products include the bench-top PSQ 96, PSQ 96MA and PSQ HS 96A Systems, all of which utilize proprietary software and reagent kits, and Rotor-Gene 3000, CAS 1200 and other products from Corbett. From an initial pool of 2300 items drawn from the literature, a population survey, and professional experience, several versions of the PSQ have been developed, researched, and modified (Wilkin et al. |
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