Pas1Pulmonary Adenoma Susceptibility 1
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A 285-bp 5'-phosphorylated dsDNA substrate for the enzyme was first generated from the pUC19 (New England Biolabs) DNA template by a standard PCR process with the 5'-phosphorylated primers (MWG Biotech) PS1 (5'-P-000GAAAGGGGGATGTGCTGCAAGG-3') and PAS1 (5'-PGTGAGCGCAACGCAATTAATGTGAG-3') and Taq polymerase supplied by Qiagen Ltd.
We then amplified 5[micro]L of each reaction mixture in a standard "detection" PCR process using the PS1 and PAS1 primers.
The Standard Physician Global Assessment (PGA) rating system was utilized for clinical response on the trunk and limbs for the 4-week study, in addition to a modified PAS1 (Psoriasis Area Severity Index) outcome measure (mPASI) for the scalp evaluation.