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3 mol/L sodium acetate), 5x Denhart solution (50x Denhardt solution consists of 1% wt/vol Ficoll 400 (Sigma), 1% wt/vol polyvinylpyrrolidone (Sigma), and 1% wt/vol bovine serum albumin (Sigma, Fraction V), 0. 5 mg/mL each final concentration of Ficoll, polyvinylpyrrolidone, and bovine serum albumin; Sigma-Aldrich Corp. 4,642,267, 4,769,013, 6,203,812, and 6,395,289 which relate to Hydromer's AQUAMERE(R) technology, a unique combination of polyurethane and polyvinylpyrrolidone to which an anti-infective agent can be added. |
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