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Although real-time PCR or pyrosequencing is more rapid than high-throughput assays for mutation detection (4,5), the conventional PCR technique can be applied to detect drug-resistant mutation (6) in areas lacking real-time PCR or pyrosequencing capabilities. 454 Life Sciences, an innovative developer of genome-scale sequencing technologies and a majority-owned subsidiary of CuraGen Corporation (NASDAQ: CRGN), has obtained an exclusive license for sole use of sequencing by synthesis and pyrophosphate based sequencing for whole genome applications from Pyrosequencing AB (Stockholm: PYRO A), a global technology solutions provider for the Applied Genomics market. He co-founded Pyrosequencing in 1997 (renamed to Biotage in 2003) and ParAllele BioScience in 2001 (sold to Affymetrix). |
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