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PCR products were excised from agarose gel, purified with QIA quick gel extraction kit (Qiagen Inc. Briefly, PCR products were purified by using the QIA quick PCR Purification Kit (QIAGEN GmbH) according to manufacturer's instruction. PCR products were purified by using agarose gel electrophoresis and a QIA quick gel extraction kit (Qiagen Ltd, Crawley, West Sussex, England) and sequenced by using a Beckman Coulter CEQ 2000 capillary sequencer and CEQ 2000 Dye Terminator cycle sequencing Quick Start kit (Beckman Coulter, Fullerton, CA). |
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