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In addition, recombinant sequences were confirmed in patients' samples by using the reverse transcription polymerase chain reaction (RT-PCR) with primers RECF (5'-CTCAACAGAGCTGTGCTAGTCATGCAATCC-3'; nucleotide positions 5229-5258) and RECR (5'-TCCACTGAGGTTGAGAAAACCATATTGCAC-3'; nucleotide positions 5748-5777), designed on the basis of isolates SHA63 and SHA66 genome sequences and DNA sequencing. Peckham said, "Roman Mica was selected for this interview on RECR not only because of his innovations with eMailaCard, but also because of his creative ideas for creating professional visibility. |
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