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One to five million PBMCs were added to 1 mL RLT buffer (Qiagen, Valencia, CA) containing 1% [beta]-mercaptoethanol to preserve RNA in the cells. Samples were incubated overnight at 56[degrees]C in 80 [micro]L of proteinase K with 5 mL of Buffer RLT from the Rneasy Midi kit and then processed per manufacturer's directions. 3 kg (45 lb) Job Simulation Visit POD RLT MLC 1 8 9-kg (20-lb) Unable to completely lift box, 1 x 10 11. |
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