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RNasin |
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4 mmol/L of each deoxynucleoside triphosphate, 10 U RNasin RNase Inhibitor (Promega, Madison, WI, USA), 20 pmol of the genomic and reverse primers, 1 [micro]L enzyme mix (containing Omniscript and Sensiscript Reverse Transcriptases and HotStarTaq DNA polymerase) and 2. 2], 10 mM dithiothreitol (DTT), and 6 U RNasin in a total volume of 25 [micro]L. Synthesis of cDNA was done in a 20-[micro]L reaction volume containing 2 mmol/L deoxynucleotide triphosphates, 20 U of RNAsin (Promega, Madison, WI, USA), 10 pmol of random hexamer, 200 U of Moloney murine leukemia virus reverse transcriptase (Promega), 4 [micro]L of 5 x RT buffer, and 5 [micro]L of extracted RNA. |
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