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5 mL) containing blood agar made from defibrinated rabbit blood overlaid with a 1:1 mixture of RPMI 1640 medium and Schneider Drosophila cell culture medium supplemented with 10% fetal calf serum (Sigma, Saint Louis, MO, USA, and Gibco-BRL, Gaithersburg, MD, USA), 10,000 IU penicillin (Biotika, L'upca, Slovakia), 100 [micro]g/mL amikacin (Bristol-Myers Squibb, Princeton, NJ, USA), and 1,500 [micro]g/mL 5-fluorocytosine (Sigma). Can I substitute minimal essential media for our RPMI 1640 and still get good growth? CV-1, Hep-2, LLC-Mk2, MEK, and RK-13 cells were maintained in 199 medium with 5% FBS, and A549 cells were maintained in RPMI 1640 medium supplemented with 10% FBS. |
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