Both the reference method tests and the ADA and the RTPCR
hybridization probes for M.
In addition, due to the slight overlap of the emission spectra of the dyes, the software's color compensation function was applied during the analysis of all RTPCR
The quality of Culicoides RNA was assessed by SYBR green real-time PCR (Quantitect SYBR Green RTPCR
kit, Qiagen, CA) of the Culicoides sonorensis actin gene CS-Act1D-F686, TATGCCTTACCACATGCT ATCC and Act1D-R805, AATTTCACGTTCGGC AGTTG (10).
La reaccion de RT-PCR se llevo a cabo utilizando un estuche comercial que incluye las enzimas SuperScript[R] III One-Step RTPCR
System y Platinum[R] Taq DNA Polymerase (Invitrogen), de acuerdo al protocolo de la casa comercial.
It is possible by exploiting more sensitive expression analyzing methods like Real-Time RTPCR
alaSOS4 changes by exogenous ABA would be detectable.
Moreover the virus was detected by both RTPCR
and isolation in cell culture in 2 specimens that were collected day 9 post onset of parotitis.
CDC protocol of realtime RTPCR
for influenza A (H1N1) http://www.
Viral RNA was extracted from allantoic luid by TRIzol (Gibco-BRL, USA) method and reverse transcription was performed with random primers or influenza A specific universal primer (12) and SuperScript III RTPCR
system (Invitrogen, USA).
HO-1 and GST-pi protein and mRNA levels were determined by Western blot and RTPCR
The overall diagnostic accuracies of RIDT and DFA, compared with RTPCR
, were 76% and 88%, (Pediatrics 2010 Feb.
They then translated the classifier into an RTPCR
platform with 79 genes that showed high accuracy (>90%) in classifying 5 tissue classes and applied it to 13 CUP cases.
Relative expression by real-time RTPCR
of the pil genes is novel.